This product is a set of two tablets - one DAB tablet and one Urea Hydrogen Peroxide tablet. When prepared according to instructions, the solution should be used within one hour for best results.
产品名称
SIGMAFAST™ 3,3′-二氨基联苯胺片, tablet, To prepare 5 mL
表单
tablet
储存温度
−20°C
SMILES字符串
Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI key
HSTOKWSFWGCZMH-UHFFFAOYSA-N
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应用
开发用于免疫组织学和免疫印迹，作为过氧化物酶活性定位的沉淀底物。DAB 是免疫组织学的首选底物，因为其可产生对酒精具有抗性的强烈棕黑色染料。用 DAB 染色的载玻片可脱水，封片于树脂介质中，并储存以备将来引用。SIGMAFAST™ DAB 片剂不需要额外的缓冲液或步骤来制备活性底物溶液。片剂单独包装在铝箔包装中
重悬
将每片片剂溶于 5 mL 去离子水中，得到含 DAB 和过氧化氢尿素的即用型缓冲液。
其他说明
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Hello, your product D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for how many months after dissolving in 5ml of distilled water?
1 answer-
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Hello, Can https://www.sigmaaldrich.com/US/en/product/sigma/d4293?icid=sharepdp-clipboard-copy-productdetailpage this product be used to develop western blots? Thank you, Regards Vanda
1 answer-
D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for WB on PVDF membranes, please see attached reference paper stating below: "The PVDF was then collected and rinsed 3 times for 5–10 min each wash with TBS. The blot was then developed appropriately with 3',3-Diaminobenzidine tablets for developing peroxidase (D4293 Sigma-Aldrich) and BCIP/NBT for alkaline phosphatase (B5655 Sigma-Aldrich) and the blot was imaged."Scientific Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells Neus Gomila Pelegri1,2, Bruce K. Milthorpe1 , Catherine A. Gorrie2 , Jerran Santos1. Stem Cell Investig 2023;10:7
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