You can use MTT to assess the intactness of isolated mitochondria. For this purpose, the CT01-5 Sigma-Aldrich MTT Reagent A is suitable. Additionally, the following publication may be of interest as a protocol reference: PLoS One. 2015 Jul 29;10(7):e0134139. This publication discusses the prevention of cisplatin-induced acute kidney injury and can provide valuable insights.
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品質等級
用途
kit sufficient for 200 tests (of 1 mL cell suspensions)
kit sufficient for 40 tests (of 5 mL cell suspensions)
包裝
pkg of 1 kit
儲存條件
dry at room temperature
技術
flow cytometry: suitable
protein staining: suitable
螢光
λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)
應用
cell analysis
detection
檢測方法
fluorometric
運輸包裝
wet ice
儲存溫度
2-8°C
相关类别
一般說明
在正常细胞中，JC-1染料能够聚集于线粒体基质中，形成红色的荧光聚集物。任何消耗线粒体跨膜电位的活动（如凋亡）都会影响线粒体中的JC-1染料聚集，导致这种染料分散到整个细胞中，从而使红色荧光（JC-1聚集体）转变为绿色荧光（JC-1单体）。本试剂盒可进行细胞染色，再通过荧光显微镜观察或流式细胞仪观测或测定荧光强度。
應用
线粒体电化学势梯度 (Δψ) 消耗被视为细胞凋亡的早期症状。本试剂盒采用阳离子亲脂性染料JC-1，可在活细胞中方便、快速地检测线粒体内膜电化学电位变化。
特點和優勢
试剂盒提供：
- 可简单、快速、方便地染色和分析线粒体完整性
- 包含检测线粒体内膜电化学势变化所需的全部试剂
- 包含抗生素缬氨霉素，可作为防止JC-1聚集的对照试剂
- 可在绿色和红色通道中清晰地检测到荧光变化
- 适用于贴壁细胞和悬浮细胞
- 已经过Jurkat、U-937、HeLa和NIH3T3细胞的测试
- 可通过荧光显微镜或流式细胞仪观测和分析荧光信号
仅试剂盒组分
产品编号
说明
- DMSO 1 mL
- JC Staining Buffer 5X 120 mL
- JC-1 1 mg
- Valinomycin Ready Made .1 mL
儲存類別代碼
10 - Combustible liquids
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Robert L Elliott et al.
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Frontiers in cellular and infection microbiology, 7, 439-439 (2017-10-27)
A Cossarizza et al.
Biochemical and biophysical research communications, 197(1), 40-45 (1993-11-30)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
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氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
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