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Merck
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EMT6/VRP/R

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC代码:
41106514
价格与库存信息目前不能提供

生物来源

mouse unknown/unspecified

包装

tube of 5 μg 96042319-DNA-5UG
pkg of vial of cells 96042319-1VL

生长模式

Adherent

核型

Not specified

形态学

Epithelial-like

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Mouse mammary, drug-resistant

细胞系描述

The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in verapamil (see reference).

应用

Drug-resistance studies

培养基

MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 μg/ml verpamil + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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