ATHOS

93122325, Porcine thyroid, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: ATHOS, 93122325
• 生物源: Porcine thyroid
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Thyroglobulin, plasminogen activator (u-PA)
• 受體: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Porcine thyroid epithelial

細胞系描述

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

應用

Mechanism of thyroid follicle reconstruction

培養基

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

其他說明

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