BE10-7

93040125, rat brain, Not specified

• UNSPSC代码: 41106514

属性

• 产品名称: BE10-7, 93040125
• 生物来源: rat brain
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Not specified
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat, BDIX, brain, pre-malignant

细胞系描述

Clone of the line BE10-Intermediate (Sigma Catalogue number. 93040119). The cells have elevated levels of tissue plasminogen activity but do not grow in agar or in BDIX rats. The cells should not be used beyond passage 24 as they start to form tumours.

应用

Study of carcinogenesis

培养基

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C.

其他说明

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