Detroit 573

90112601, human skin, Fibroblast-like

• UNSPSC代码: 41106514

属性

• 产品名称: Detroit 573, 90112601, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• 生物来源: human skin
• 包装: tube of 5 μg 90112601-DNA-5UG; pkg of vial of cells 90112601-1VL
• 生长模式: Adherent
• 核型: Aneuploid
• 形态学: Fibroblast-like
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian skin, B/D translocation

细胞系描述

Detroit 573 was initiated in 1967 from a skin biopsy of a deceased new-born female with multiple congenital anomalies. Approximately 94% of the cell population is 45,XX,t(Bp-;Dq+). The translocated B/D chromosome morphologically resembles a group A-2 chromosome. The cell line is susceptible to polyvirus type 1 and vesicular stomatitis (Indiana Strain) virus.

应用

Karyology studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.