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product name
VH2, from Daboia russelii cardiovascular, 90102539, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
生物源
Daboia russelii cardiovascular
增長模式
Adherent
染色體組型
2n = 34
形態學
Fibroblast-like
受體
Not specified
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細胞系來源
Viper Russells heart
細胞系描述
Derived from the heart of a normal female Russell′s viper (Daboia russelii). Unlike cell line VSW the cells do not produce C type particles.
培養基
EMEM (HBSS) (M5775) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + fetal bovine serum (FBS / FCS) (Heat Inactivated).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 28-30°C.
其他說明
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