M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• UNSPSC代码: 41106514

属性

• 产品名称: M-MSV-BALB/3T3, 90030802
• 生物来源: mouse embryo
• 生长模式: Adherent
• 核型: Modal no. 57
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

细胞系描述

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

应用

Transformation and virus studies: M-MSV, MuLV

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.