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product name
180BR, 90011862
生物源
human skin
增長模式
Adherent
染色體組型
Not specified
形態學
Fibroblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human skin fibroblast
細胞系描述
Established from a biopsy of a radiosensitive patient. The patient was diagnosed as not having ataxia-telangiectasia, an autosomal recessive disorder where sensitivity to ionising radiation has been documented.
應用
DNA repair studies
培養基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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