U-87 MG Cell Line human

89081402, human brain (glioblastoma astrocytoma), Epithelial-like

• 别名: U-87MG Cells, U87 Cells, U87MG Cells
• 分類程式碼代碼: 41106514

属性

• 产品名称: U-87 MG Cell Line human, from human brain(glioblastoma astrocytoma), 89081402
• 生物源: human brain (glioblastoma astrocytoma)
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human glioblastoma astrocytoma

細胞系描述

Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.

應用

Neural studies

U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma.[1] It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.[2]

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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