RAG

89040605, mouse kidney, Ameboid-like

• 分類程式碼代碼: 41106514

属性

• product name: RAG, 89040605
• 生物源: mouse kidney
• 增長模式: Adherent
• 染色體組型: Modal no. 68, hypertriploid
• 形態學: Ameboid-like
• 產品: ES-2
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c renal adenocarcinoma

細胞系描述

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

應用

Hybridisation, resistance and tumourigenicity studies

培養基

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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