UT-1

87100701, hamster ovary, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: UT-1, 87100701
• 生物来源: hamster ovary
• 生长模式: Adherent
• 核型: 2n = 22
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Hamster Chinese ovary

细胞系描述

Derived from parent line CHO-K1. Cells were exposed to increasing concentrations of compactin over 8 months. During this exposure the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase synthesis increased 500-fold. The enzyme controls cholesterol synthesis.

应用

Enzymatic studies

培养基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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