NIH-3T3D4

85111801, mouse embryo, Fibroblast

• UNSPSC代码: 41106514

属性

• 产品名称: NIH-3T3D4, 85111801
• 生物来源: mouse embryo
• 包装: tube of 5 μg 85111801-DNA-5UG; pkg of vial of cells 85111801-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

一般描述

Product has lost contact inhibition

细胞系来源

Mouse Swiss NIH embryo

细胞系描述

Derived from the NIH-3T3 cell line. No longer exhibit contact inhibition.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2x10,000 to 2x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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