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product name
TR6Bc1, 85042301
生物源
mouse nerve
增長模式
Adherent
染色體組型
Not specified
形態學
Glial
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse C3H.He Schwannoma
細胞系描述
Ethyl nitrosourea induced tumour in C3H. The mice, originated in trigeminal nerve and brain; readily transplanted and resembles schwannoma histologically. Cultured cells form prominent processes, resemble 33B (Sigma Catalogue number. 85081901) morphologically, contain abundant C type particles.
應用
Tumourigenicity studies
培養基
DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他說明
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