推荐产品
product name
ONE58 cell line human, 10092313
生物源
human esophagus
描述
Human malignant mesothelioma
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial-like: cells are spindle-shaped with few vacuoles.
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human malignant mesothelioma
細胞系描述
This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143
應用
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
培養基
RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS
例行更新培養
Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
其他說明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
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