LA-N-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6041202, human nerve, Large elongated cells + small round cells in clusters

• UNSPSC代码: 41106514

属性

• 生物来源: human nerve
• 包装: tube of 5 μg 06041202-DNA-5UG; pkg of vial of cells 06041202-1VL
• 生长模式: Not specified
• 核型: Modal no. 73, range 46 - 73
• 形态学: Large elongated cells + small round cells in clusters
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Neuroblastoma

细胞系描述

Established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The culture consists mostly of large, elongated cells with processes with some small round cells adhering to the elongated cells. The cells are tumourigenic in nude mice.

培养基

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

传代培养常规

For routine maintenance, split cultures after they have become dense i.e. once every 3-4 weeks at a 1:20 to 1:50 ratio; 8% CO₂; 37oC. The culture consists of large elongated cells with processes and small round cells. The latter tend to grow in dense clusters on the former and detach readily. Most cells are moderately adherent. Remove attached cells from the substrate with trypsin/EDTA. Cells will detach in 5-10 minutes. When resuscitated from a frozen ampoule cells may appear dead after a day but reattach and resume growth within 2-3 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes.

其他说明

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