P3X63Ag8.653-TurboDoma

3092502, mouse blood, Lymphoblast

• 分類程式碼代碼: 41106514

属性

• product name: P3X63Ag8.653-TurboDoma, 03092502
• 生物源: mouse blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c myeloma, serum-free

細胞系描述

P3X63Ag8.653 cell line (Sigma Catalogue number. 85011420) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma^™. Suitable for use as a fusion parent for serum-free generation of hybridomas.

應用

Fusion partner for generation of serum-free hybridomas.

培養基

TurboDoma^™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

例行更新培養

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

其他說明

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法律資訊

TurboDoma is a trademark of Cell Culture Technologies