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Merck

C4129

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

别名:

α-chymotrypsin A and B, alpha-chymotrypsin

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
42010112
NACRES:
NA.54

種類

Type II

形狀

lyophilized powder

比活性

≥40 units/mg protein

分子量

25 kDa

成份

protein, ≥85%

顏色

white to off-white

UniProt登錄號

應用

diagnostic assay manufacturing

儲存溫度

−20°C

基因資訊

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應用

sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系,使用固定化金属离子亲和层析(IMAC)和固定化金属离子亲和毛细管电泳(IMACE)。

生化/生理作用

α-胰凝乳蛋白酶是丝氨酸肽酶,并且具有通过二硫键连接的三条多肽链(A 链-13 残基,B 链-131 残基和 C 链-97 残基)中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca2+ 激活和稳定酶。该酶被二异丙基氟磷酸盐(DFP)、甲苯磺酰氟(PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK)、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu2+ 和 Hg2+ 抑制。
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。

單位定義

一个单元将在 pH 7.8,25℃下,每分钟水解 1.0 μmole BTEE。

準備報告

以 3×结晶的胰凝乳蛋白酶原为原料生产

分析報告

通过 E1%/280 确定的蛋白质

其他說明

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Invasion of human red blood cells (RBCs) by Plasmodium parasites is a crucial yet poorly characterised phenotype. Two-color flow cytometry (2cFCM) promises to be a very sensitive and high throughput method for phenotyping parasite invasion. However, current protocols require high
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Postharvest storage of legumes is a strategy to ensure food security. However, the hard-to-cook phenomenon, partly explained by pectin-cation-phytate theory, may develop during storage. Based on this theory, minerals could be redistributed within the matrix, affecting the concentration of free/bound
K Y Jiang et al.
Biochimica et biophysica acta, 1433(1-2), 198-209 (1999-08-14)
Chemical glycosylation of bovine alpha-chymotrypsin, by a glucosamine adduct on the carboxyl group, results in the modification of its catalytic activity. The structural alterations of alpha-chymotrypsin resulting from its glycosylation are studied by immobilized metal-ion affinity chromatography (IMAC) and immobilized
Robert Winkler
Rapid communications in mass spectrometry : RCM, 24(3), 285-294 (2010-01-06)
Electrospray ionization (ESI) ion trap mass spectrometers with relatively low resolution are frequently used for the analysis of natural products and peptides. Although ESI spectra of multiply charged protein molecules also can be measured on this type of devices, only

商品

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

Chromatograms

application for HPLC

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