The activity of this enzyme is established using a 0.02 M Tris buffer with a starting pH of 8.3 at 0°C. One unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. However, the optimum pH and temperature is highly dependent on the specific application. There are a number of publications regarding the working parameters for this material. Please see the link below to review an example:
https://pubmed.ncbi.nlm.nih.gov/10701452/#:~:text=It%20was%20determined%20that%20the,61706%20Dalton%20by%20gel%20filtration.
推荐产品
生物来源
bovine erythrocytes
方案
≥75% protein basis (biuret)
≥95% (SDS-PAGE)
表单
lyophilized powder
比活
≥3,500 W-A units/mg protein
溶解性
deionized water: >10 mg/mL
储存温度
2-8°C
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生化/生理作用
碳酸酐酶是一种含锌的酶，催化二氧化碳可逆地转化为碳酸氢盐。它的主要生理作用之一是维持血液和其他组织中的酸碱平衡。碳酸酐酶缺乏导致碳酸酐酶 II 类型缺乏综合征，是一种常染色体隐性遗传疾病，可引起骨硬化症、肾小管酸中毒和脑钙化 [3]。
单位定义
一个 Wilbur-Anderson (W-A) 单位将导致 0.02 MTrizma 缓冲液的 pH 值在 0°C 下从 8.3 降至 6.3/min（一个 W-A 单位基本上相当于一个 Roughton-Booth 单位）。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Nicolas Ghéczy et al.
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Enzyme-catalysed cascade reactions in flow-through systems with immobilised enzymes currently are of great interest for exploring their potential for biosynthetic and bioanalytical applications. Basic studies in this field often aim at understanding the stability of the immobilised enzymes and their
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Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds, e.g., luteolin 5-O-β-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-β-primeveroside (7), and 4-(β-d-glucopyranosyloxy)benzyl 3,4-dihydroxybenzoate
Marco Catalano et al.
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The availability of reliable methods for the characterization of the binding of small molecule ligands to protein targets is crucially important for drug discovery. We have adapted a method, routinely used for the characterization of monoclonal antibodies (enzyme-linked immunosorbent assay
K M Gilmour et al.
The Journal of experimental biology, 212(Pt 11), 1647-1661 (2009-05-19)
Carbonic anhydrase (CA) is the zinc metalloenzyme that catalyses the reversible reactions of CO(2) with water. CA plays a crucial role in systemic acid-base regulation in fish by providing acid-base equivalents for exchange with the environment. Unlike air-breathing vertebrates, which
Joana da Costa Ores et al.
Chemosphere, 88(2), 255-259 (2012-04-20)
This work presents a study of industrially applicable techniques to obtain a biologically supported carbon dioxide capture system, based on the extraction of carbonic anhydrase from bovine blood. Carbonic anhydrase is a metalloenzyme which catalyzes the reversible hydration of carbon
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What is recommand usage temperature and pH range?
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What is the recommended storage conditions for "Carbonic Anhydrase, from bovine erythrocytes?
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The recommended storage conditions for Solution of Carbonic Anhydrase, from bovine erythrocytes may be frozen at –20 °C for future use. It is advised to avoid repeated freezing and thawing. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2–3 uses.
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What is the isozyme I or isozyme II of Carbonic Anhydrase from bovine erythrocytes (C2624 Carbonic Anhydrase)?
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The product C2624, Carbonic Anhydrase from bovine erythrocytes, consists mainly of CA II. However, there are also small levels of CA I and CA IV present.
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Is it possible to obtain information about the type of Carbonic Anhydrase (I or II) included in item C2624-carbonic anhydrase?
1 answer-
Product C2624, Carbonic Anhydrase from bovine erythrocytes, with a purity of ≥95% (SDS-PAGE), consists predominantly of CA II, with small levels of CA I and CA IV present.
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