生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 26-30 kDa
物種活性
mouse, human, rat
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human colon adenocarcinoma sections
microarray: suitable
western blot: 1:2,000 using human Burkit lymphoma Raji cell lysate
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... BCL2L1(598)
mouse ... Bcl2l1(12048)
rat ... Bcl2l1(24888)
一般說明
Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x, long isoform (Bcl-x) gene is mapped to human chromosome 20q11.21. BCL2L1 belongs to the Bcl-2 family. The bcl-x gene on alternative splicing leads two distinct proteins. It comprises multiple Bcl-2 homology (BH3) domains. Bcl-xL is expressed in the adult brain.(10)
特異性
The antibody reacts with Bcl-xL.
免疫原
synthetic peptide corresponding to the N-terminal of human Bcl-x (amino acids 2-20) conjugated to BSA. This sequence is identical in human and mouse Bcl-xS, Bcl-xL, Bcl-Xβ and Bcl-Xγ isoforms and is highly conserved in rat Bcl-xS and Bcl-xL (single amino acid substitution).
應用
生化／生理作用
Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x arelong isoforms (Bcl-x) of proteins that are involved in regulating apoptosis. Similar to Bcl-2, Bcl-xL can inhibit cell death upon growth factor removal, when transfected in an IL-3 dependent cell line. Bcl-xL can form ion channels in biological membranes and may maintain cell survival by regulating the permeability of intracellular membranes. Bcl-xL may bind to apoptotic protease activating factor 1 (Apaf-1) preventing it from activating caspase 9. Like Bcl-2, Bcl-x activity is regulated by specific phosphorylation of a 60-amino acid regulatory loop region. Bcl-xL has been shown to regulate cell response to oxidative stress, cellular resistance to DNA damage-induced apoptosis by chemotherapeutic agents and ionizing radiation, and favors neurite outgrowth.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Katherine Amps et al.
Nature biotechnology, 29(12), 1132-1144 (2011-11-29)
The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late
Chloe F A Warren et al.
Cell death & disease, 10(3), 177-177 (2019-02-23)
The BCl-2 family has long been identified for its role in apoptosis. Following the initial discovery of BCL-2 in the context of B-cell lymphoma in the 1980s, a number of homologous proteins have since been identified. The members of the
Jianfang Wang et al.
Cellular signalling, 23(12), 2030-2038 (2011-08-16)
Functional analysis of a Bcl-xL phosphorylation mutant series has revealed that cells expressing Bcl-xL(Ser49Ala) mutant are less stable at G2 checkpoint after DNA damage and enter cytokinesis more slowly after microtubule poisoning, than cells expressing wild-type Bcl-xL. These effects of
A J Minn et al.
Nature, 385(6614), 353-357 (1997-01-23)
Bcl-2-related proteins are critical regulators of cell survival that are localized to the outer mitochondrial, outer nuclear and endoplasmic reticulum membranes. Despite their physiological importance, the biochemical function of Bcl-2-related proteins has remained elusive. The three-dimensional structure of Bcl-xL, an
Megan Stevens et al.
Frontiers in genetics, 10, 804-804 (2019-09-26)
Apoptosis plays a vital role in cell homeostasis during development and disease. Bcl-x, a member of the Bcl-2 family of proteins, is a mitochondrial transmembrane protein that functions to regulate the intrinsic apoptosis pathway. An alternative splicing (AS) event in
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