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生物源
mouse
共軛
biotin conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
SPA-27, monoclonal
形狀
buffered aqueous solution
技術
direct ELISA: 1:80,000-1:160,000
同型
IgG1
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
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一般說明
Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
特異性
对蛋白A具有特异性，与蛋白G无交叉反应性。与游离蛋白A或IgG结合蛋白A反应，不干扰蛋白A的Fc结合活性。
免疫原
来自金黄色葡萄球菌Cowan I菌株的蛋白A。
應用
Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.
生化／生理作用
Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.
準備報告
Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.
儲存和穩定性
Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Antibodies for Immunohistochemistry
Immunohistochemistry: Basics and Methods (2009)
Single-domain protein A-engineered magnetic nanoparticles: toward a universal strategy to site-specific labeling of antibodies for targeted detection of tumor cells
Mazzucchelli S, et al.
ACS Nano, 4(10), 5693-5702 (2010)
Protein A chromatography: challenges and progress in the purification of monoclonal antibodies
Ramos-de-la-Pena AM, et al.
Journal of Separation Science (2019)
Evaluation of indirect enzyme-linked immunosorbent assays and IgG avidity assays using a protein A-peroxidase conjugate for serological distinction between Brucella abortus S19-vaccinated and-infected cows
Pajuaba ACAM, et al.
Clinical and Vaccine Immunology : CVI, 17(4), 588-595 (2010)
Manasi Balachandran et al.
Virulence, 9(1), 390-401 (2017-11-22)
Staphylococcus pseudintermedius is an opportunistic pathogen in dogs and the most frequent cause of canine pyoderma. Protein A, a potent virulence factor in S. aureus is encoded by the spa gene. S. pseudintermedius possesses genes seemingly analogous to spa, but
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