A8353

Anti-hABH3 antibody, Mouse monoclonal

clone hABH3-99, purified from hybridoma cell culture

• 别名: Anti-Human AlkB Homologue 3, Monoclonal Anti-hABH3 antibody produced in mouse
• MDL编号: MFCD06412252
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: mouse
• 偶联物: unconjugated
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: hABH3-99, monoclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~33 kDa
• 种属反应性: rat, hamster, monkey, bovine, canine, human, mouse
• 技术: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of 293T cells
• 同位素/亚型: IgG1
• UniProt登记号: Q96Q83
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: Monkey ... ALKBH3(715921) ; human ... ALKBH3(221120) ; mouse ... Alkbh3(69113) ; rat ... Alkbh3(362169)

说明

一般描述

Human alkB homolog 3, alpha-ketoglutarate dependent dioxygenase (hABH3) is mainly found in the nucleoplasm and cytoplasm.[1] Anti-hABH3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH3-99 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with hABH3, conjugated to keyhole limpet hemocyanin (KLH).

特异性

Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.

应用

• Mouse Monoclonal Anti-hABH3 has been used in:
• western blot
• immunohistochemistry[1]
• immunoprecipitation
• indirect enzyme linked immunosorbent assay (ELISA)

Mouse Monoclonal Anti-hABH3 can be used for western blot (1-2μg/mL), immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies.

生化/生理作用

Human alkB homolog 3, α-ketoglutarate dependent dioxygenase (hABH3) is involved in the repair of alkylated RNA. This antibody serves as an important tool for studying the regulation of DNA/RNA repair enzymes.

hABH3 is a Fe(II)-dependent dioxygenase involved in the repair of alkylation-induced damage in DNA. hABH3 mutations have been analysed in pediatric brain tumors.
Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: nwg
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable