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Merck

A6982

Sigma-Aldrich

HydroMatrix细胞培养多肽支架

mixture, powder

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About This Item

分類程式碼代碼:
12352207

生物源

synthetic

品質等級

形狀

powder

儲存溫度

−20°C

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應用

HydroMatrix 已被证明可在许多细胞类型培养中促进细胞生长和迁移,包括神经干细胞、神经元、胶质细胞、星形胶质细胞、成纤维细胞和角质形成细胞。

成分

HydroMatrix 是一种肽纳米纤维三维支架,可促进细胞生长和迁移。当使用高温或高离子强度时,它利用从流体前体自组装成高度交联的肽水凝胶的特定肽。通过诱导从溶液到凝胶的快速转化,生成肽纳米纤维支架。调整 HydroMatrix 溶液的浓度,研究人员可以控制三维架构的灵活性,以满足其应用的特定需求。

注意

该冻干品置于 -20℃ 下,在该温度下可稳定多年。水凝胶应储存于 -70°C,使有效期最大化。1%(w/v)储备溶液应储存在 -20°C,并可稳定 3-6 个月。

準備報告

该产品以冻干粉形式提供。加入无菌水可以制备 10mg/mL 的 1%(w/v)储备溶液,得到刚性凝胶。0.5%(w/v)溶液将产生更柔软的凝胶,更适合细胞迁移和血管生成。0.15%-0.25% 范围内的较低浓度更适用于神经元等多种细胞类型。在水溶液中,HydroMatrix 的 pH 值为 2.5,如果没有事先添加缓冲液或培养基,则不应直接添加到细胞中。一旦形成水凝胶,不要试图进一步混合凝胶,因为这有可能破坏水凝胶的完整性。操作细胞时应小心谨慎,以免破坏支架。

法律資訊

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Amish Asthana et al.
Drug discovery today, 17(15-16), 810-817 (2012-04-10)
The three microenvironmental factors that characterize 3D cultures include: first, chemical and/or biochemical composition, second, spatial and temporal dimensions, and third, force and/or substrate physical properties. Although these factors have been studied individually, their interdependence and synergistic interactions have not
Daniela Stoppoloni et al.
Rheumatology international, 33(9), 2399-2403 (2012-03-28)
Osteoarthritis (OA) is one of the most common degenerative joint disease for which there is no cure. It is treated mainly with non-steroidal anti-inflammatory drugs to control the symptoms and some supplements, such as glucosamine and chondroitin sulphate in order
Amish Asthana et al.
Drug discovery today, 18(11-12), 533-540 (2012-12-29)
Force and substrate physical property (pliability) is one of three well established microenvironmental factors (MEFs) that may contribute to the formation of physiologically more relevant constructs (or not) for cell-based high-throughput screening (HTS) in preclinical drug discovery. In 3D cultures
Josine E G Vaes et al.
Glia, 69(3), 655-680 (2020-10-13)
Encephalopathy of prematurity (EoP) is a common cause of long-term neurodevelopmental morbidity in extreme preterm infants. Diffuse white matter injury (dWMI) is currently the most commonly observed form of EoP. Impaired maturation of oligodendrocytes (OLs) is the main underlying pathophysiological

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