推荐产品
生物源
rabbit
品質等級
共軛
alkaline phosphatase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous glycerol solution
物種活性
mouse
技術
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000 using using β-Actin in total cell extract of HeLa cells (5-10 μg/well)
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid, capable of fighting bacterial and viral infection.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
免疫原
Purified mouse IgG.
應用
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in immunocytochemical localization of Aβ peptides and western blot analysis.
Anti-mouse IgG (whole molecule)-alkaline phosphatase antibody can be used in immunocytochemistry.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.
生化／生理作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
外觀
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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