After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.
推荐产品
形狀
powder
品質等級
技術
activity assay: suitable
基質
cross-linked dextran
基質活性組
functional group
珠尺寸
40-125 μm (dry)
孔徑
~30,000 Da exclusion limit
工作pH值
2-9
容量
3-4 meq/g ion exchange capacity
相容性
mode of use mixed weak and strong anion exchange chromatography
一般說明
Sephadex® 离子交换剂含有具有官能团的交联葡聚糖基质。这些基团通过稳定的醚键连接到基质中的葡萄糖单元。DEAE Sephadex 可用于离子交换色谱法纯化和分离蛋白质。
A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02
A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02
應用
DEAE® Sephadex A-25 已用于：
- 形成 DEAE Sephadex A-25（甲酸盐形式），用于使用辣根植物提取硫代葡萄糖苷（GLS）
- 甘蓝芽中 GLS 含量的测定
- 星形胶质细胞制备和纯化恩多泽平
- 在急性心肌梗死（AMI）患者中鉴定和标记胰岛素激活的一氧化氮合酶抑制剂（蛋白）
特點和優勢
- 弱阴离子交换剂
- 适用于批处理技术
- 高结合能力
- 对常用的水性缓冲剂添加剂（如 8M 尿素和 6M 盐酸胍）无反应
法律資訊
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
T M Parkinson
Journal of lipid research, 8(1), 24-29 (1967-01-01)
Various cellulose and dextran anion exchangers bind bile salts in vitro under conditions of pH and ionic strength resembling those in the lumen of the small intestine. Of these substances, diethylaminoethyl (DEAE) cellulose, guanidoethyl cellulose, and DEAE Sephadex reduced hypercholesterolemia
Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Hohn, D., et al.
Immunological Investigations, 12(5), 481-490 (1983)
A C Newton et al.
Analytical biochemistry, 156(1), 56-60 (1986-07-01)
The greatly enhanced fluorescence of Tb3+ when complexed with dipicolinic acid affords a simple and highly sensitive method for monitoring continuous anion flux through the erythrocyte anion transporter, band 3. Dipicolinic acid (pyridine-2,6-dicarboxylic acid) is entrapped in human erythrocytes and
In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
P H Jones
Experimental cell research, 139(2), 401-404 (1982-06-01)
H Wang et al.
Neuroscience, 46(4), 793-806 (1992-01-01)
We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified
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How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should I store Product A25120, DEAE-Sephadex® A-25 chloride?
1 answer-
Unused media can be stored in the container at +4 to +30 °C. Ensure that the screw-top is fully tightened. Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.
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How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.
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What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.
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