The specific composition of the elution buffer E7777 is considered proprietary. However it is a Tris-HCL solution without EDTA.
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用途
sufficient for 70 purifications
技术
DNA purification: suitable
储存温度
15-25°C
一般描述
GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。
DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后，再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA，至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(<40 bp)。
纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。
DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后，再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA，至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(<40 bp)。
纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。
应用
纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。
特点和优势
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可在8分钟内纯化出多达100 μL或10 μg 的PCR扩增产物DNA。
- 可去除99%以上的引物和其他成分
- 可在8分钟内纯化出多达100 μl或10 μg 的PCR扩增产物DNA。
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可去除99%以上的引物和其他成分
- 不需要去除有机萃取引入的矿物油
- 相对于其他市场领先竞争对手，可多获得40%的纯化制备物
其他说明
GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。
原理
GenElute PCR回收试剂盒将硅胶结合和微量离心形式的优势结合在一起。DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后，再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA，至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(可去除<40 bp的片段)。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Warning
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Genetic analysis of uveal melanoma by array comparative genomic hybridization before and after radiotherapy
Werner Wackernagel
Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry, 27.6, 286-291 (2013)
Genetic identification of intestinal microsporidia species in immunocompromised patients in Tunisia.
Najla Chabchoub et al.
The American journal of tropical medicine and hygiene, 80(1), 24-27 (2009-01-15)
Stool samples from 86 immunocompromised patients (51 human immunodeficiency virus (HIV)-infected patients and 35 patients with haematologic malignancies) were systematically screened for intestinal microspordiosis by microscopic examination and polymerase chain reaction (PCR) using universal primer V1/PMP2. Nine samples (10.5%) showed
Adriana P Rebelo et al.
Nucleic acids research, 37(20), 6701-6715 (2009-09-11)
To characterize the organization of mtDNA-protein complexes (known as nucleoids) in vivo, we have probed the mtDNA surface exposure using site-specific DNA methyltransferases targeted to the mitochondria. We have observed that DNA methyltransferases have different accessibility to different sites on
Charlotte G Cole et al.
Genome biology, 9(5), R78-R78 (2008-05-15)
Although the human genome sequence was declared complete in 2004, the sequence was interrupted by 341 gaps of which 308 lay in an estimated approximately 28 Mb of euchromatin. While these gaps constitute only approximately 1% of the sequence, knowledge
Andreas Veith et al.
Frontiers in microbiology, 2, 37-37 (2011-07-13)
The sulfur oxygenase reductase (SOR) is the initial enzyme of the sulfur oxidation pathway in the thermoacidophilic Archaeon Acidianus ambivalens. The SOR catalyzes an oxygen-dependent sulfur disproportionation to H(2)S, sulfite and thiosulfate. The spherical, hollow, cytoplasmic enzyme is composed of
实验方案
GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
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What is the elution buffer made of? As it can be replaced with water, is it just water that is provided?
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Does this kit remove proteins from PCR product?
1 answer-
This is a post-pcr clean up kit. The single or double-stranded amplification products are bound to the silica membrane. Then all non-DNA material such as primers, nucleotides, and polymerase are washed away. Finally the purified product is eluted. Anything that isn't bound will be removed.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
1 answer-
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
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Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
1 answer-
Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
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Can Product No. NA1020, GenElute™ PCR Clean-Up Kit, be used to clean up PCR products larger than 10 kb?
1 answer-
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
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When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
1 answer-
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
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When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
1 answer-
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
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