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M1028

Sigma-Aldrich

氯化镁 溶液

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

别名:

MgCl2, dichloromagnesium, magnesium chloride

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10 X 1 ML
$96.00
100 ML
$131.00

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10 X 1 ML
$96.00
100 ML
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About This Item

线性分子式:
MgCl2
CAS号:
7786-30-3
分子量:
95.21
MDL编号:
MFCD00011106
UNSPSC代码:
12352302
PubChem化学物质编号:
24896582
NACRES:
NA.52
等级:
for molecular biology
表单:
liquid
溶解性:
water: soluble

$96.00

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等级

for molecular biology

质量水平

300

无菌性

non-sterile; 0.2 μm filtered

表单

liquid

浓度

1.00 M±0.01 M

技术

DNA sequencing: suitable

杂质

DNAse, none detected
 NICKase, none detected
 Protease, none detected
 RNAse, none detected

溶解性

water: soluble

密度

1.080 g/cm3

痕量阳离子

heavy metals (as Pb): ≤5 ppm

适用性

suitable for electrophoresis
suitable for molecular biology

应用

cell analysis

异质活性

DNase, RNase, NICKase and protease, none detected

SMILES字符串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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相关类别

一般描述

氯化镁是一种常见的离子型卤化物，易溶于水。它在酶学、细胞膜/细胞壁结构、肌肉细胞生理学和核酸结构中起多种重要的生物学作用。此外，氯化镁在许多酶中都是基本的辅酶，包括脱氧核糖核酸酶、某些限制性酶和核糖核酸酶H。氯化镁在各种分子生物学应用中（包括PCR反应）广泛用于提供镁离子。

应用

氯化镁在各种分子生物学应用，包括PCR反应和缓冲液中提供镁离子。用途：
  • 配制免疫沉淀实验的细胞裂解缓冲液[1]
  • 加入Krebs-Henseleit缓冲液，用于老年小鼠的长期高脂饮食研究。[2]
  • 用于三维微生物转谷氨酰胺酶水凝胶中的细胞透化，进行骨细胞分化及互连细胞网络的体外研究。[3]
  • 保护肝脏免遭氧化损伤。[4]
  • 制备无血清培养基，用于大鼠脑切片培养物的电生理研究。[5]

特点和优势

  • 即用型溶液，免去配制步骤，节约时间。
  • 重金属（以铅计）：≤5 ppm
  • 不含DNA酶、RNA酶、切口酶和蛋白酶

组分

氯化镁溶液是含有1M氯化镁的18兆欧水。

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000429332
0000429788
0000429788
0000429332
0000408966

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实验方案

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

变溶菌素的酶促检测

在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。

己糖激酶的酶促检测

在测定己糖激酶活性时，使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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