跳转至内容
Merck
所有图片(6)

主要文件

CMC0017

Sigma-Aldrich

过表达 ™ C41 (DE3) 化学感受态细胞

Escherichia coli, rod shaped

别名:

C41 strain

登录查看公司和协议定价

选择尺寸

20 X 40 μL
$907.00

$907.00

请联系客服了解存货情况
所有图片(6)

选择尺寸

变更视图
20 X 40 μL
$907.00

About This Item

UNSPSC代码:
41106202
NACRES:
NA.85

$907.00

请联系客服了解存货情况

产品名称

过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression

生物来源

Escherichia coli

等级

for molecular biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

运输

dry ice

储存温度

−70°C

相关类别

一般描述

OverExpress™ 电感受态和化学感受态细胞是可以有效表达所有种类生物体（包括真细菌、酵母、植物、病毒和哺乳动物）的有毒蛋白质的大肠杆菌。这些新菌株表达有毒蛋白质的有效性已在350多篇论文中得到验证。OverExpress™菌株含有从表型上选择的基因突变，以产生对有毒蛋白质的耐受性。C41(DE3) 菌株来源于 BL21(DE3)。该菌株至少有一处突变，其防止了与许多重组有毒蛋白表达相关的细胞死亡。菌株C43（DE3）来源于C41（DE3），是通过选择对不同毒性蛋白的抗性而得到的，并且可以表达一组不同的C41（DE3）的毒性蛋白。OverExpress C41（DE3）pLysS和C43（DE3）pLysS还带有抗氯霉素的质粒，该质粒可编码T7溶菌酶，其是T7 RNA聚合酶的一种天然抑制剂。含有pLysS的细胞会产生少量的T7溶菌酶。这些菌株用于抑制诱导以前T7 RNA聚合酶的基础表达，从而使编码特别有毒的蛋白质的重组体保持稳定。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

应用

过表达 ™ C41 (DE3) 化学感受态细胞已用于表达筛选。[1]

特点和优势

  • 用这些 BL21 (DE3) 衍生物克隆到任何 T7 载体的表达基因
  • 有效表示毒性和膜蛋白类
  • 被超过 350 篇研究文章引用

组分

  • 过表达 C41 (DE3) 化学感受态细胞
  • pUC 19 转化对照 DNA
  • 表达回收培养基


法律信息

OverExpress is a trademark of Imaxio, S.A.

储存分类代码

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCQ1390
SLCM4781
SLCM5528
SLCL5983
SLCJ4341

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Lily Goodyer Sait et al.
eLife, 9 (2020-10-23)
Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium
A high-throughput expression screening platform to optimize the production of antimicrobial peptides.
Schreiber C, et al.
Microbial cell factories, 16(1), 29-29 (2017)
Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in
Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair.
Altin Sula et al.
Molecular cell, 81(6), 1160-1169 (2021-01-28)
Voltage-gated sodium channels are targets for many analgesic and antiepileptic drugs whose therapeutic mechanisms and binding sites have been well characterized. We describe the identification of a previously unidentified receptor site within the NavMs voltage-gated sodium channel. Tamoxifen, an estrogen
查看所有相关文献

实验方案

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

Questions

Reviews

No rating value

Active Filters

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门