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Merck

93773

Sigma-Aldrich

TWEEN ® 20

viscosity 250-450 mPa.s (25 °C)

别名:

聚氧乙烯山梨糖醇酐单月桂酸酯, 聚氧乙烯脱水山梨醇单月桂酸酯

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250 G
$82.90
1 KG
$161.00

$82.90


国内现货,预计发货时间2025年4月03日详情


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250 G
$82.90
1 KG
$161.00

About This Item

CAS号:
MDL编号:
UNSPSC代码:
12161900
NACRES:
NA.21

$82.90


国内现货,预计发货时间2025年4月03日详情


获取大包装报价

描述

non-ionic

表单

viscous liquid

分子量

~1228

技术

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

折射率

n20/D 1.468 (lit.)
n20/D 1.471

粘度

250-450 mPa.s(25 °C)

HLB值

16.7±1.0

密度

1.11 g/mL at 20 °C
1.095 g/mL at 25 °C (lit.)

SMILES字符串

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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一般描述

吐温® 20 是聚氧乙烯山梨糖醇酯,属于聚山梨醇酯家族。它是一种分子量为 1,225 道尔顿的非离子型洗涤剂,假设有 20 个环氧乙烷单元,1 个山梨糖醇和 1 个月桂酸作为主要脂肪酸。环氧乙烷亚单元负责表面活性剂的亲水性,而烃链提供疏水环境。山梨糖醇形成与环氧乙烷聚合物连接的骨架环。[1]

应用

Tween® 20(吐温20)广泛用于多种生化应用。它已被用于:

  • 作为用于制备稳定的水包油乳液的乳化剂[2]
  • 在膜的预提取中去除外周蛋白(以 2% 的浓度,用于提取膜结合蛋白)[3]
  • 作为基于膜的免疫分析的封闭剂,典型浓度为 0.05%- 1%[4][5][6]
  • 裂解哺乳动物细胞,浓度 0.005 至 0.5%
  • 原位杂交之前再水化组织[7]
非离子洗涤剂

生化/生理作用

Tween® 20(吐温20)用作去污剂,作用原理是通过结合水不溶性物质的疏水部分令其变得亲水。

特点和优势

  • 适合蛋白纯化、DNA和RNA提取
  • 适合哺乳动物细胞培养
  • 高纯度产品,适合研究使用

注意

储存时会变混浊,加热后澄清

其他说明

如需了解生化试剂系列的更多信息,请填写此表

法律信息

TWEEN is a registered trademark of Croda International PLC

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

527.0 °F - Pensky-Martens closed cup

闪点(°C)

275 °C - Pensky-Martens closed cup

个人防护装备

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Enhanced p62 expression triggers concomitant autophagy and apoptosis in a rat chronic spinal cord compression model.
Chen Z, et al.
Molecular Medicine Reports, 9(6), 2091-2096 (2014)
Reynolds JEF
Martindale: The Extra Pharmacopoeia, 1030-1030 (1993)
Temporal expression of cytokines and signal transducer and activator of transcription factor 3 activation after neonatal hypoxia/ischemia in mice.
Snapkov I, et al.
Developmental Neuroscience, 35(2-3), 212-225 (2013)
The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis.
Snapkov I, et al.
BMC Cancer, 16(1), 490-490 (2016)
Detergents: An Overview.
Neugebauer JM
Methods in Enzymology, 182, 239-253 (1990)

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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