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Merck

92011

Sigma-Aldrich

Nitrile hydratase

recombinant, expressed in E. coli, ≥1.5 U/mg

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About This Item

CAS号:
分類程式碼代碼:
12352204

重組細胞

expressed in E. coli

形狀

lyophilized

比活性

≥1.5 U/mg

儲存溫度

−20°C

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應用

Nitrile hydratase is an enzyme that is used to convert nitriles to amides. It is used to study the metabolism of nitriles in bacteria, to produce acrylamide, and to remove nitriles from wastewater . Product 92011 is used to convert acrylonitrile to acrylamide.

生化/生理作用

Nitrile hydratase catalyses the hydration of nitriles to their corresponding amides. Nitrile hydratase is composed of α- and β-subunits. It has a non-heme iron or a noncorrin cobalt ion in a ligand environment that includes two oxidized cysteine residues .

包裝

Bottomless glass bottle. Contents are inside inserted fused cone.

單位定義

1 U corresponds to the amount of enzyme which converts 1 μmol acrylonitrile to acrylamide per minute at 30°C and pH 7.2

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Zhemin Zhou et al.
The Journal of biological chemistry, 284(22), 14930-14938 (2009-04-07)
The incorporation of cobalt into low molecular mass nitrile hydratase (L-NHase) of Rhodococcus rhodochrous J1 has been found to depend on the alpha-subunit exchange between cobalt-free L-NHase (apo-L-NHase lacking oxidized cysteine residues) and its cobalt-containing mediator (holo-NhlAE containing Cys-SO(2)(-) and

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