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化驗
~70%
形狀
solid
溶解度
H2O: soluble
螢光
λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+
適合性
suitable for fluorescence
儲存溫度
−20°C
SMILES 字串
Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1
InChI
1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)
InChI 密鑰
OZLGRUXZXMRXGP-UHFFFAOYSA-N
應用
Fluo-3和相关分子Fluo3/AM可用作细胞内钙离子(Ca2+)的荧光指示剂。Fluo-3可用于流式细胞术以及使用可见光激发的共焦激光扫描显微术(与在488nm下操作的氩激光源兼容)。与钙结合后,荧光强度增加约40倍。
其他說明
用于流式细胞术的钙离子荧光指示剂。与钙结合后,荧光强度增加约40倍
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Journal of immunological methods, 127(2), 197-205 (1990-03-09)
Cytoplasmic free calcium [( Ca2+]i) is a key intracellular messenger in many cell types. We have used fluo-3, a recently developed calcium probe, to study [Ca2+]i in resting and stimulated human peripheral blood T lymphocytes. The spectral properties of fluo-3
Contact dermatitis, 81(3), 184-193 (2019-04-23)
Retinoic acid (RA)-induced dermatitis is the most frequent side-effect limiting its widespread use. However, the exact mechanisms triggering dermatitis are not fully understood, including the role of skin mast cells. The newly discovered Mas-related G-protein-coupled receptor-X2 (MRGPRX2) in mast cells
Cell death and differentiation, 26(9), 1600-1614 (2018-11-06)
Mitochondrial superoxide dismutase 2 (SOD2) is a major antioxidant defense enzyme. Here we provide evidence that SOD2 plays critical roles in maintaining calcium homeostasis in newly generated embryonic cerebral cortical neurons, which is essential for normal mitochondrial function and subcellular
Acta pharmacologica Sinica, 39(5), 875-884 (2018-03-30)
Xyloketal B (Xyl-B) is a novel marine compound isolated from mangrove fungus Xylaria sp. (No 2508). We previously showed that Xyl-B promoted endothelial NO release and protected against atherosclerosis through the Akt/eNOS pathway. Vascular NO production regulates vasoconstriction in central
Food & function, 10(8), 4661-4673 (2019-07-12)
Hydroxysafflor yellow A (HSYA) is the main active ingredient of edible plant safflower. HSYA has demonstrated anti-inflammatory effects. The inflammatory response is the key mechanism responsible for asthma, and the pro-inflammatory platelet-activating factor (PAF) is known to play a role
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