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Merck
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文件

45245

Sigma-Aldrich

曙红5-异硫氰酸酯

≥95.0% (UV)

别名:

EITC

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About This Item

经验公式（希尔记法）:
C21H7Br4NO5S
CAS号:
60520-47-0
分子量:
704.96
MDL號碼:
MFCD00082502
分類程式碼代碼:
12352200
PubChem物質ID:
57650268

化驗

≥95.0% (UV)

螢光

λex 521; λem 544 (pH 9.0)

儲存溫度

2-8°C

SMILES 字串

OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S

InChI

1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)

InChI 密鑰

MCXYFYWNOWPDMA-UHFFFAOYSA-N

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應用

曙红5-异硫氰酸酯（EITC）是一种荧光探针。在荧光共振能量转移（FRET）测定中，EITC标记的分子可与荧光素-5′-异硫氰酸酯（FITC）标记的分子一起使用。

其他說明

受体荧光团。测定蛋白质的旋转扩散;用于荧光能量转移研究

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317 - H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Yan Fu et al.
Journal of immunological methods, 289(1-2), 57-64 (2004-07-15)
A solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) for the determination of human complement3 (C3) based on a sandwich type assay and a labelled avidin-biotin (LAB) type assay was described. The anti-human complement3 and avidin were labeled with eosin5-isothiocyanate. On
J W Erickson et al.
Biochemistry, 34(27), 8693-8700 (1995-07-11)
In this work, we have used resonance energy transfer to determine the relative positions of a reactive cysteine residue on the gamma subunit of the retinal cyclic GMP phosphodiesterase (gamma PDE) and a reactive lysine residue on the alpha subunit
Photohemolysis of human erythrocytes labeled in band 3 with eosin-isothiocyanate.
J P Pooler et al.
Photochemistry and photobiology, 44(4), 495-499 (1986-10-01)
A D Horowitz et al.
Biochemistry, 32(37), 9513-9523 (1993-09-21)
The self-association of pulmonary surfactant protein SP-C in lipid vesicles was studied using fluorescence energy transfer. Bovine SP-C was labeled with two fluorescent probes, succinimidyl 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoate and eosin isothiocyanate, on the amino terminus of the protein, producing NBD-SP-C and EITC-SP-C
Ya-lan Wu et al.
Guang pu xue yu guang pu fen xi = Guang pu, 24(12), 1597-1600 (2005-04-15)
In this paper, the medium (pH, organic solvent and surfactant) effects on fluorescence of eosin-5-isothiocyanate (eosin-ITC) have been studied in detail. The results show that the pH and polarity of system studied are two great important influence factors on fluorescence
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