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儲存溫度
−20°C
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一般說明
iTRAQ®试剂是首个多重、胺特异性、稳定同位素试剂组合，可同时标记8种不同生物学样本中的所有多肽以进行鉴定及相对和绝对定量，并且保留重要的翻译后修饰(PTM)信息。
包含iTRAQ® 试剂113、114、115、116、117、118、119及121，以及SDS变性剂、TCEP还原剂、MMTS半胱氨酸封闭试剂、TEAB缓冲液和异丙醇等，可用于最初的实验方案开发。
该试剂盒包含与Sciex iChemistry® 货号4390811相同的试剂。
该试剂盒包含与Sciex iChemistry® 货号4390811相同的试剂。
應用
iTRAQ® Reagent - 8PLEX One Assay Kit蛋白质多重检测试剂盒已用于相对和绝对定量同位素标记(iTRAQ, isobaric tag for relative and absolute quantitation)：
- 芥菜芽总蛋白消化样本
- 西兰花芽总蛋白
- 来源于不同植物/完整叶绿体的脱盐多肽
特點和優勢
- 同时对多个样本中的蛋白质进行鉴定和定量
- 标记所有肽段以扩大蛋白和蛋白组覆盖率，包括翻译后修饰(PTM)分子
- 标记每个蛋白的多个肽，提高MS/MS质谱鉴定和定量的可靠性
- 提高结果通量和可靠性，便于进行蛋白质生物标志物研究
- 流程简化，无需进行样品分离以降低样本复杂性，比如蛋白质亲和沉降技术(affinity pull-down)
- 一次实验最多可同时检测8种不同的生物样本
- ProteinPilot软件全面支持所有的SCIEX蛋白组学LC/MS/MS平台
分析報告
法律資訊
iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.
訊號詞
Danger
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 3
閃點（°C）
2.0 °C - closed cup
其他客户在看
Xiaoming Ma et al.
Frontiers in veterinary science, 8, 663031-663031 (2021-09-18)
The plateau adaptability and stress resistance of yaks are widely known based on their capacity to survive under severe habitat conditions. However, a few studies on brain mitochondria have characterized these adaptations at the protein level. We identified and quantified
Chao Cheng et al.
RSC advances, 10(10), 6052-6062 (2020-02-06)
Heat stress has been proved to increase the content of melatonin in plants. In the present study, a combination of methods including physiological and biochemical, gene transcription and proteomic were used to investigate the melatonin accumulation mechanisms in mustard sprouts
Peitong Wang et al.
Plant physiology, 178(4), 1568-1583 (2018-10-13)
Arsenic (As) is highly toxic to plants and detoxified primarily through complexation with phytochelatins (PCs) and other thiol compounds. To understand the mechanisms of As toxicity and detoxification beyond PCs, we isolated an arsenate-sensitive mutant of Arabidopsis (Arabidopsis thaliana), arsenate
Yongqi Yin et al.
RSC advances, 11(20), 12336-12347 (2022-04-16)
Exogenous melatonin (10 μM) enhances ZnSO4 (4 mM) stress tolerance and regulates the isothiocyanate content of broccoli sprouts. Nevertheless, the molecular mechanism underlying the role of melatonin in isothiocyanate metabolism under ZnSO4 stress is unclear. The effects of exogenous melatonin
Amber E Alsop et al.
Nature communications, 6, 6841-6841 (2015-04-17)
During apoptosis, Bak permeabilizes mitochondria after undergoing major conformational changes, including poorly defined N-terminal changes. Here, we characterize those changes using 11 antibodies that were epitope mapped using peptide arrays and mutagenesis. After Bak activation by Bid, epitopes throughout the
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