This medium does not contain VEGF.
产品211-500是客户指定产品，不在网上销售。 联系客户支持
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
Angel Tan et al.
Biomaterials science, 7(8), 3460-3470 (2019-07-04)
In the research field of nanostructured systems for biomedical applications, increasing attention has been paid to using biomimetic, dynamic cellular models to adequately predict their bio-nano behaviours. This work specifically evaluates the biointeractions of nanostructured lipid-based particles (cubosomes) with human
Yang Li et al.
Life sciences, 232, 116590-116590 (2019-06-23)
Endothelial cell (EC) apoptosis is fundamental for the pathophysiology of atherosclerosis, in which microRNAs (miRNAs) emerge as critical regulators. miR-122 has been shown to regulate the apoptosis of various cell types, however, whether miR-122 is associated with atherosclerosis and EC
Jaya Sriram et al.
International journal of environmental research and public health, 15(10) (2018-10-24)
Airborne particulate matter (PM) exposure remains the leading environmental risk factor for disease globally. Interventions to mitigate the adverse effects of PM are required, since there is no discernible threshold for its effects, and exposure reduction approaches are limited. The
Ekramy Elmorsy et al.
Toxicology in vitro : an international journal published in association with BIBRA, 44, 154-163 (2017-07-18)
Antidepressants are commonly employed for the treatment of major depressive disorders and other psychiatric conditions. We investigated the relatively acute cytotoxic effects of three commonly prescribed antidepressants: fluoxetine, sertraline, and clomipramine on rat primary blood brain barrier endothelial cells over
Zheng Wei et al.
Cellular signalling, 44, 118-126 (2018-01-18)
Thromboangiitis obliterans (TAO) (also known as Buerger's disease) is an inflammatory vascular disease that predominantly affects small- and medium-sized blood vessels of extremities. Endothelial cells play critical roles in the initiation and progression of this disease, but the underlying mechanisms
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
实验方案
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
关于人主动脉内皮细胞解冻、传代培养和冻存的技术信息。*从液氮储罐中取出冻存管时，请务必戴上面罩和手套。从液氮储罐到房间的剧烈温度变化可以导致冻存管中存留的任何液氮爆裂并造成人员受伤。
人脐静脉内皮细胞（HUVEC）培养实验方案。储存方法。培养的准备工作。冷冻管在抵达后须立即储存在液氮储存箱中。
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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Does the medium contain VEGF? If so, can you specify the concentration?
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If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?
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This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.
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Can you please provide the full composition of the medium with the concentrations?
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This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.
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Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.
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This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.
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