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生物来源
human prostate
包装
tube of 5 μg 10112303-DNA-5UG
pkg of vial of cells 10112303-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
See Lang SH, et.al (2006)
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
细胞系来源
Prostate cancer moderately well differentiated, early stage
包装
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
DNA图谱分析
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 12,14
D7S820: 7,12
THO1: 6,9.3
TPOX: 8,12
vWA: 15,17
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 12,14
D7S820: 7,12
THO1: 6,9.3
TPOX: 8,12
vWA: 15,17
培养基
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene.
Norman J Maitland et al.
Methods in molecular medicine, 88, 275-285 (2003-11-25)
Shona H Lang et al.
In vitro cellular & developmental biology. Animal, 42(8-9), 273-280 (2006-12-14)
Three-dimensional epithelial culture models are widely used to emulate a more physiologically relevant microenvironment for the study of genes and signaling pathways. Prostate epithelial cells can grow into solid cell masses or acinus-like spheroids in Matrigel. To test if the
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