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Merck

08445

Sigma-Aldrich

7-氨基-4-甲基香豆素-3-乙酸

BioReagent, suitable for fluorescence, ~90% (HPLC)

别名:

2H-1-苯并吡喃-3-乙酸, 7-氨基-4-甲基-3-香豆素醋酸, AMCA酸

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100 MG
$132.00
500 MG
$461.00

About This Item

经验公式(希尔记法):
C12H11NO4
分子量:
233.22
Beilstein:
7927205
MDL编号:
UNSPSC代码:
12352106
PubChem化学物质编号:
NACRES:
NA.32

$132.00


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产品线

BioReagent

方案

~90% (HPLC)

表单

powder

溶解性

DMF: soluble
DMSO: soluble
aqueous base: soluble

荧光

λex 350 nm; λem 433 nm in methanol

适用性

suitable for fluorescence

SMILES字符串

CC1=C(CC(O)=O)C(=O)Oc2cc(N)ccc12
CC1=C(CC(O)=O)C(=O)Oc2cc(N)ccc12

InChI

1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

InChI key

QEQDLKUMPUDNPG-UHFFFAOYSA-N

应用

AMCA是一种用于标记蛋白的试剂。其在345 nm处具有特征吸收峰以及在440-460 nm处具有荧光发射(蓝色区域);然而,当与蛋白质缀合时,吸收峰移至355 nm,并在440-460 nm处发射荧光。其具有高的荧光产量和足够的斯托克斯位移(100 nm),并且相对光稳定 [1]。其相对容易被激活并偶联至肽的N端氨基,从而形成酸稳定的酰胺键 [2][3]

包装

无底玻璃瓶。内含物在插入的融合锥体内。

其他说明

有效的蓝色荧光团,可用于免疫荧光, λabs ~350 nm, λem ~440 nm[4]

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

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M W Wessendorf et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 38(1), 87-94 (1990-01-01)
7-Amino-4-methylcoumarin-3-acetic acid (AMCA) has been found to be a useful fluorophore for immunofluorescence. The present study describes a spectrophotometric method for determining the ratio of moles AMCA to moles protein (or the f/p ratio) in an AMCA-conjugated IgG. The concentration
C Otto et al.
The American journal of physiology, 271(1 Pt 1), C210-C217 (1996-07-01)
Dipeptide uptake was studied in primary cultures from rat anterior pituitaries by use of radiolabeled carnosine and the fluorescent dipeptide derivative beta-Ala-Lys-N epsilon-AMCA (AMCA is 7-amino-4-methylcoumarin-3-acetic acid). Fluorescence microscopic studies revealed that the reporter peptide specifically accumulated in the S-100
Melissa J Leyva et al.
Chemistry & biology, 17(11), 1189-1200 (2010-11-26)
Huntington's Disease (HD) is characterized by a mutation in the huntingtin (Htt) gene encoding an expansion of glutamine repeats on the N terminus of the Htt protein. Numerous studies have identified Htt proteolysis as a critical pathological event in HD postmortem
B Ulfhake et al.
Journal of neuroscience methods, 40(1), 39-48 (1991-11-01)
This paper describes the implementation of an ultraviolet (UV) laser (Spectra Physics 171-18 with 3 lines: 334, 351 and 364 nm in UV) as light source for fluorescence confocal scanning microscopy. With this instrument it is possible to use fluorophores
D Delia et al.
Cytometry, 12(6), 537-544 (1991-01-01)
Antibodies coupled to 7-aminocoumarin (AMCA) emit a bright blue fluorescence under ultraviolet (UV) excitation and are therefore ideal for three-color immunofluorescence (IF) with fluorescein (FITC) and phycoerythrin (PE) labeled reagents; however, due to the different absorption spectra, the use of

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