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Merck

G6257

Sigma-Aldrich

戊二醛 溶液

Grade II, 25% in H2O

别名:

1,5-戊二醛, 五碳双缩醛 溶液

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10 ML
$29.80
100 ML
$110.00
10 X 10 ML
$152.00
1 L
$292.00

$29.80


预计发货时间2025年7月15日详情


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变更视图
10 ML
$29.80
100 ML
$110.00
10 X 10 ML
$152.00
1 L
$292.00

About This Item

线性分子式:
OHC(CH2)3CHO
CAS号:
分子量:
100.12
Beilstein:
605390
MDL编号:
UNSPSC代码:
12352114
PubChem化学物质编号:
NACRES:
NA.21

$29.80


预计发货时间2025年7月15日详情


获取大包装报价

类型

Grade II

质量水平

表单

liquid

浓度

25% in H2O

技术

electron microscopy: suitable

颜色

colorless

有效pH范围

2.9

mp

-10  °C ((14 °F ))

溶解性

water: soluble

应用

cell analysis
sample preparation

SMILES字符串

[H]C(CCCC([H])=O)=O

InChI

1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2

InChI key

SXRSQZLOMIGNAQ-UHFFFAOYSA-N

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一般描述

戊二醛,也称1,5-戊二醛,是一种多功能试剂,其应用涵盖各种研究领域,包括细胞生物学和生物化学研究。它可用作蛋白质交联剂,协助显微镜学中的酶固定,有助于组织化学和细胞化学过程。作为胺反应性同型双功能交联剂,它可用作SDS-PAGE、染色或电子显微镜检查之前的细胞固定剂, 是各种形态学研究的重要工具。

戊二醛可以使胺和肼衍生物与蛋白质和其它含胺聚合物有效地交联。特别值得一提的是,它可以使生物素酰肼与核酸直接结合,其潜在应用为荧光酰肼和羟胺与DNA的偶联。另外,在组织学研究中,戊二醛可用于保存组织切片,以便进行详细检查。

应用

戊二醛溶液已被用于:
  • 固定小鼠[1] 和食道癌细胞[2]
  • 交联淀粉样β蛋白[3]
  • 在气管三维支架的制备中交联明胶[4]

生化/生理作用

戊二醛是一种有效的蛋白质交联剂,可用于酶固定化显微镜分析、组织化学和细胞化学等技术。它在酸性或中性条件下以不同形式存在。[5]它对水生生物有毒:[6]戊二醛过敏将导致过敏性反应。在内窥镜检查过程中接触戊二醛蒸气会导致结肠炎。[7]

特点和优势

  • 功能强大,适用于各种研究应用
  • 即用型溶液减少制备时间

包装

运输途中未冷藏

注意

该等级不建议用作电镜固定液。

制备说明

未经过特别纯化。

其他说明

为了全面了解我们针对客户研究提供的各种单糖产品,建议您访问我们的碳水化合物分类页面。
如需了解生化试剂系列的更多信息,请填写此表

免责声明

“根据全球生物杀灭剂的法规,包括但不限于美国环境保护局的《联邦杀虫剂、杀真菌剂和灭鼠剂法案》、《欧洲生物杀灭剂法规》、 加拿大害虫管理局、土耳其《生物杀灭剂产品法规》、韩国《消费化学产品和生物杀灭剂安全管理法案》(K-BPR),该产品不可用作生物杀灭剂。”

警示用语:

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

补充剂危害

储存分类代码

8A - Combustible corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Amyloid-beta oligomerization in Alzheimer dementia versus high-pathology controls
Esparza TJ, et al.
Annals of Neurology, 73(1), 104-119 (2013)
A novel tissue-engineered trachea with a mechanical behavior similar to native trachea
Park JH, et al.
Biomaterials, 62(1), 106-115 (2015)
Sorption characteristics of mixed molecules of glutaraldehyde from water on mesoporous acid-amine modified low-cost activated carbon: mechanism, isotherm, and kinetics
Lloyd M, et al.
Journal of Chemistry, 2015, 790-802 (2015)
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking
Migneault I, et al.
Biotechniques, 37(5), 790-802 (2004)
Initiation of apoptosis, cell cycle arrest and autophagy of esophageal cancer cells by dihydroartemisinin
Du XX, et al.
Biomedicine and Pharmacotherapy, 67(5), 417-424 (2013)

Questions

1–5 of 5 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is the 25% concentration in terms of w/w or w/v?

    1 answer
    1. This product is a 25% weight per weight (w/w) solution.

      Helpful?

  4. What type of product should be ordered to ensure that each lot provides clear glutaraldehyde solutions?

    1 answer
    1. Grade II glutaraldehyde solutions may exhibit variation in color between lots. For consistent clarity, it is advisable to order Grade I glutaraldehyde, which is a purer product. This higher-grade variant must be stored frozen and is shipped with wet or dry ice. While Grade II is often suitable for industrial or technical applications, Grade I glutaraldehyde solutions are designed for more precise applications, such as electron microscopy, where a pure product is essential.

      Helpful?

  5. What is the storage?

    1 answer
    1. This product may be stored at ambient temperature. This material is air-sensitive and should be stored under inert gas to preserve product integrity.

      Helpful?

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