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P1379

Sigma-Aldrich

TWEEN ® 20

TWEEN® 20
2 of 4 reviewers received a sample product or took part in a promotion

viscous liquid

别名:

聚氧乙烯山梨糖醇酐单月桂酸酯, 聚氧乙烯脱水山梨醇单月桂酸酯

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25 ML
$23.60
100 ML
$53.00
250 ML
$75.20
500 ML
$97.20
1 L
$127.00
4 L
$272.00

$23.60

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25 ML
$23.60
100 ML
$53.00
250 ML
$75.20
500 ML
$97.20
1 L
$127.00
4 L
$272.00

About This Item

CAS号:
9005-64-5
MDL號碼:
MFCD00165986
分類程式碼代碼:
12161900
NACRES:
NA.21

$23.60

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描述

non-ionic

品質等級

300

化驗

>40% (GC)

形狀

viscous liquid

分子量

~1228

成份

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

濃度

≥40.0% (GC)

技術

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

雜質

≤3.0% water

折射率

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

酸值

≤2.2 mg/g

羥值

96‑108 mg/g

轉變溫度

cloud point 76 °C

密度

1.095 g/mL at 25 °C (lit.)

HLB

16.7

適合性

complies for IR spectroscopy

SMILES 字串

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI 密鑰

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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一般說明

Tween®20是属于聚山梨醇酯家族的一种聚氧乙烯山梨糖醇酯。它是一种分子量为1,225道尔顿的非离子型去污剂，假设有20个环氧乙烷单位、1个山梨糖醇和1个月桂酸作为主要脂肪酸。环氧乙烷亚基负责表面活性剂的亲水性，而烃链则提供了疏水环境。山梨糖醇可形成连接了环氧乙烷聚合物的骨架环。[1]
吐温® 20 是一种非离子表面活性剂和去污剂，广泛应用于生物化学和生命科学研究。其主要用途涉及膜-蛋白复合物分离过程中膜蛋白的增溶。 此外，它可作为各种清洗、封闭、稀释和稳定缓冲液的成分。另外，它可用作免疫组织化学（包括蛋白质印迹和ELISA等技术）的试剂。在这些应用中，吐温® 20起到关键作用，例如防止非特异性抗体结合，最大程度减少背景染色，确保试剂的均匀分布。

表面活性剂同时具有亲水和疏水成分，可以分为两大类：在水中电离的离子型（阴离子、阳离子或两性）和不电离的非离子型 。所述产品为非离子表面活性剂，可用于各种应用，如乳化、分散、增溶和去污剂。此外，它可用作膜蛋白的增溶剂。

應用

TWEEN 20作为组分已用于：
  • Western blotting中的洗涤和封闭缓冲液[2]
  • 免疫组化中的封闭缓冲液[3]
  • ELISA（酶联免疫吸附测定）中的洗涤缓冲液[4][5]
  • PCR（聚合酶链反应）中的反应混合液[6]
TWEEN 20是一种广泛用于生化应用的非离子型去污剂。它已被作为乳化剂用于稳定的水包油乳液的制备。TWEEN 20已用于膜的预提取以去除外周蛋白（以2％用于膜结合蛋白的提取）。
通常0.05%浓度的TWEEN 20在基于膜的免疫检测中被用作封闭剂。0.005至0.5%的TWEEN 20可用于裂解哺乳动物细胞。
非离子洗涤剂

特點和優勢

  • 用于生命科学和生物化学研究的多功能表面活性剂
  • 良好的增溶、分散和乳化剂
  • 适用于DNA & RNA 提取、哺乳动物细胞培养和蛋白质纯化

包裝

P1379-1KT：
每个试剂盒包含3 x 100ML样品，每份样品都有唯一的制造商批号。

準備報告

TWEEN 20可与水混溶（100mg/mL），产生透明的黄色溶液。它还可与酒精、二恶烷和乙酸乙酯进行混溶。TWEEN 20不溶于液体石蜡和固定油。

其他說明

如需了解生化试剂系列的更多信息，请填写此表。

法律資訊

TWEEN is a registered trademark of Croda International PLC

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

527.0 °F - Pensky-Martens closed cup

閃點（°C）

275 °C - Pensky-Martens closed cup

個人防護裝備

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000330677
0000398258
0000398258
WXBF5137V
0000387945

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Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification.
Seal SE et al
Applied and Environmental Microbiology, 58(11), 3759-3761 (1992)
Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.
Kerwin BA
J. Pharm. Pharm. Sci., 97(8), 2924-2935 (2008)
Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle.
Gr?nevik E et al
The journal of gene medicine, 7(2), 218-227 (2005)
Ethylene and carbon dioxide production by developing strawberries show a correlative pattern that is indicative of ripening climacteric fruit.
Lannetta PM et al
Physiologia Plantarum, 127.2, 247-259 (2006)
Thomas Beuchert Kallehauge et al.
Scientific reports, 7, 40388-40388 (2017-01-17)
Recombinant protein production coopts the host cell machinery to provide high protein yields of industrial enzymes or biotherapeutics. However, since protein translation is energetically expensive and tightly controlled, it is unclear if highly expressed recombinant genes are translated as efficiently
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实验方案

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4 Reviews This action will navigate to reviews.

Questions

1–10 of 15 Questions  

  1. What is the storage temperature of Tween 20?

    1 answer

    1. This product may be stored at room temperature.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. What is the minimum dilution ratio of Tween20 in Exosome incubating buffer based on CMC level?

    1 answer

    1. This product is not tested for any specific application. The critical micelle concentration of TWEEN 20 has been reported as 60 milligrams per liter. Please see the link below to review a publication that may be helpful:
      https://open.clemson.edu/cgi/viewcontent.cgi?article=5274&context=all_theses

      Helpful?

  5. Please provide the information on product container and material for P1379-500ML TWEEN® 20

    1 answer

    1. This item is packaged in a 500 ML HDPE Bottle with a red PP cap w/ PTFE liner.

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  6. You state 0.06 mM as the CMC for this product. What is the stock concentration (in Molar) for this product? Alternatively what is the % to reach that 0.06 mM / CMC concentration? Thank you

    1 answer

    1. The critical micelle concentration (CMC) reported on the product page is a value reported from the literature. This product is a neat or pure liquid. The base molecular weight of this material is 1,225 daltons and the density is 1.095 g/mL. However, this is based on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid. Please see the link below to review the general Tween 20 datasheet, note that the CMC value listed is 60 mg/L.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/128/958/p9416pis.pdf

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  7. What is the grade/agency of Tween 20?

    1 answer

    1. This product is a research grade TWEEN 20.

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  8. What is the expiry date once opened?

    1 answer

    1. Long-term stability studies have not been performed, however, providing that the product is stored and handled properly, the expected shelf life is not expected to change. Factors such as improper closure and cross-contamination would certainly impact the overall stability and shelf life.

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  9. The data sheet for my lot says this is 50% pure. Does that mean it is 50% tween 20?

    1 answer

    1. The 50% is referring to the concentration of lauric acid which occurs in the highest concentration. Fatty acid constituents of this product are determined by transesterification to yield fatty acid methyl esters, which are identified by gas chromatography. Fatty acids that are typically present in the offered Tween products are not a quantitative measurement. These include myristic, palmitic, and stearic acid, for the most part. While the Tween is considered a 'pure' product, the molecular weight is calculated on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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1–10 of 15 Questions  

Reviews

2 of 4 reviewers received a sample product or took part in a promotion
1–2 of 4 Reviews  

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  1. Agadir Morocco
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Emulsifying properties of Polysorbates in aqueous media : applications to essential and vegetable oils

    The use of essential or vegetable oils in aqueous media requires the use of emulsifying agents such as polysorbates.
    In this review, we will focus on the properties of polysorbates in particular Tween 20 and the capacities of these agents in the emulsification of oils in aqueous media as well as the various industrial applications and in the fields of scientific research.

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    • Reviews 2
    • Vote 1
    5 out of 5 stars.

    M.Sc.

    We use the Product for surface cleaning of artworks. Works as planned.

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  3. 2 Ratings-Only Reviews

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