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應用
Girard′s reagent T may be used as a derivatizing reagent for the determination of 5-Formyl-2′-deoxyuridine in cellular DNA samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS) technique. Reducing saccharides can be derivatized by Girard′s reagent T and this can be analyzed by matrix-assisted laser desorption/ionization coupled to time of flight mass spectrometry (MALDI-TOF-MS) technique.
特點和優勢
- Girard′s reagent T is a chemical derivatization reagent for LC-MS.
- It has carbonyl functional group possessing high proton (cation) affinity that stabilizes a positive charge.
- It is commonly used in the derivatization of nucleosides.
- Derivatization reactions in mass spectrometry are used to improve ionization efficiency.
- Derivatization can enhance precise quantitative analysis for profiling of relatively small analyte molecules, particularly in metabolomics.
法律資訊
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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Nucleoside 5-formyl-2'-deoxyuridine (FodU) is a major thymidine lesion generated by reactive oxygen species. In vitro and in vivo replication studies revealed that FodU can be mutagenic. A reliable and sensitive quantification method is, therefore, important for assessing the biological implications
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