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方案
>98% (HPLC and SDS-PAGE)
表单
suspension
包装
pkg of 15 mL (05015979001 [settled resin volume])
pkg of 2 mL (11719408001 [settled resin volume])
pkg of 5 mL (11134515001 [settled resin volume])
制造商/商品名称
Roche
容量
>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)
储存温度
2-8°C
一般描述
重组蛋白A在蛋白A琼脂糖生产中的应用具有以下优点:它完全不含已知具有促有丝分裂作用的葡萄球菌肠毒素(分离自金黄色葡萄球菌的蛋白A中的污染物) 。固定化学可在广泛的pH和缓冲条件下提供高IgG的结合能力以及防漏稳定性。根据洗脱条件,凝胶可以使用30次以上。
蛋白A泄漏:<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98%纯度,HPLC,SDS-Page/1ml凝胶
蛋白A,固定化。
蛋白A泄漏:<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98%纯度,HPLC,SDS-Page/1ml凝胶
蛋白A,固定化。
特异性
蛋白A是从特定细菌菌株中分离的细胞壁蛋白,其具有来自不同物种的某些类免疫球蛋白的特异性结合位点。蛋白A可在不同程度结合IgM、IgA、IgD和大多数IgG亚类。
应用
组分
Preswollen凝胶,即用型,2ml或5ml(沉淀凝胶体积)悬浮于缓冲液中的凝胶,该缓冲液中含有20%乙醇作为保藏剂,非无菌。
蛋白A含量:3mg/ml预溶胀凝胶
蛋白A含量:3mg/ml预溶胀凝胶
质量
纯度:> 98%(HPLC,SDS-PAGE);完全不含葡萄球菌肠毒素。
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语:
Warning
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
102.2 °F
闪点(°C)
39 °C
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