跳转至内容
Merck
所有图片(3)

主要文件

UFC5030

Millipore

Amicon® Ultra过滤器,30 kDa MWCO

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa

别名:

离心浓缩器,30 kDa MWCO,0.5mL样品体积

登录查看公司和协议定价


About This Item

分類程式碼代碼:
41104916
eCl@ss:
36100101
NACRES:
NB.24
价格与库存信息目前不能提供

材料

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

品質等級

無菌

non-sterile

產品線

Amicon®

製造商/商標名

Amicon® Ultra

參數

0.5 mL sample volume

技術

protein extraction: suitable
protein purification: suitable

長度

4.99 cm (1.96 in.)

直徑

10.8 mm

過濾面積

1 cm2

容積

0.5 mL

孔徑

30 kDa MWCO

運輸包裝

ambient

一般說明

Amicon® Ultra-0.5离心过滤器可提供快速超滤功能,具有高浓缩系数,易于从稀释和复杂的样品矩阵中回收浓缩物。例如,1 mg/ml初始浓度的卵白蛋白(45 kDa)通常截留>95%。

垂直设计和可用滤膜表面积可实现快速样品处理、高样品回收率(通常超过稀释起始溶液的90%)以及30倍浓度。垂直设计可最大限度地减少溶解物极化及随后的滤膜污染,并且过滤装置中的物理停止可防止旋转至干燥及潜在样品损失。收集滤液后,可通过反向旋转快捷操作高效回收浓缩样品(保留品种)。

Amicon® Ultra-0.5装置未经灭菌处理,仅供一次性使用。Amicon® Ultra 0.5 mL过滤器的垂直滤膜设计和反向旋转功能可在更短的旋转时间内实现较高的性能。

應用

  • 采用30,000 Dalton分子量截留Ultracel®滤膜超滤
  • 浓缩含抗原、抗体、酶、核酸或微生物的生物样品
  • HPLC之前,纯化在组织培养基提取物或细胞裂解物中发现的高分子组分、去除引物、连接子或标记反应混合物中的高分子并去除蛋白质
  • 脱盐、缓冲液更换和蛋白透析等

特點和優勢

  • Ultracel®再生纤维素滤膜配有30 kDa NMWCO,其保留物回收率超过90%
  • 浓缩500 μL至15 μL
  • 浓缩系数25至30倍
  • 样品回收率高(稀释起始溶液超过90%)
  • 处理时间快:10至30分钟
  • 反向旋转功能,确保一致的回收率

聯結

替代品:42411

外觀

色码:透明

法律資訊

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10-13 - German Storage Class 10 to 13


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

商品

市售蛋白标记试剂盒让研究人员可以灵活定制自己的免疫检测panel。

Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Amicon Pro 让蛋白纯化更简单。此系统可提供一致的样品制备,可实现更可靠的蛋白回收率、同时保证纯度、简化数据生成。

查看所有结果

实验方案

如要从少量(0.5-10 mL)样品中纯化抗体,推荐使用Amicon® Pro纯化系统。

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

连续洗滤法清除蛋白或核酸样品中的去垢剂,即在过滤的同时连续补加不含去垢剂的缓冲液,保持样品体积恒定。

Questions

1–3 of 3 Questions  
  1. What is the maximum speed of an Amicon Ultra 2 device?

    1 answer
    1. The maximum speeds for concentration spins as per the User Guide are as follows:
      • Swinging bucket rotor: 4,000 × g
      • Fixed angle rotor: 7,500 × g
      It is important to note that when spinning viscous solutions like undiluted serum or plasma, the maximum speed should not exceed 5,400 x g.

      Helpful?

  2. what is the pore sizes of 30kDa, 50kDA, and 100 kDa filters? And their corresponding maximum and minimum volume which can be used?

    1 answer
    1. These ultrafiltration do not have defined pore sizes. They are characterized by retention of globular proteins, and assigned nominal pore ratings. These MWCO membrane types are found in centrifugal devices with 70, 15, 4, 2, and 0.5 ml volume capacities.

      Helpful?

  3. Can I use Amicon® Ultra Centrifugal Filter, 30 kDa MWCO to measure human serum protein binding for a small moelcule?

    1 answer
    1. The Centrifree® Ultrafiltration Centrifugal Filters are recommended for this application over the Amicon® Ultra Centrifugal Filters. Please see the link below:
      https://www.sigmaaldrich.com/product/mm/4104

      Helpful?

Reviews

No rating value

Active Filters

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门