Products TMS-008 and 2003999 are similar products. Product TMS-008 is presented as a 40mM solution at pH 4.1 +/- 0.1.
Product 2003999 is also a 40mM solution with pH range 4.1 +/- 0.1. It is also a kit component of Product ECM815.
TMS-008
Alizarin-Red Staining Solution
Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).
别名:
Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain
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About This Item
分類程式碼代碼:
12352207
NACRES:
NA.75
价格与库存信息目前不能提供
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品質等級
濃度
40 mM in distilled water
技術
cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable
一般說明
Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.
應用
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Osteobiology
Arthritis
Osteobiology
Arthritis
品質
Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.
外觀
Product is presented as a 40mM solution at pH 4.1 +/- 0.1.
儲存和穩定性
Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
新型无血清、无异种人间充质干细胞（MSC）骨细胞分化培养基的开发。
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
实验方案
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
有关间充质的信息，特别是间充质干细胞培养实验方案用于间充质干细胞（MSC）分离、扩增和分化的分步细胞培养方案。
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
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What difference between products TMS-008 Alizarin-Red Staining Solution and 2003999 Alizarin Red Solution?
1 answer-
Helpful?
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Dear Sir/Madam, Hope you are well. We already ordered ARS from your company. But we unfortunately lost the protocol inside the box. I was wondering if yo can email us the protocol. Best Regards, Ebrahim
1 answer-
Regarding your inquiry about TMS-008-C Alizarin-Red Staining Solution, there is currently no protocol available on sigmaaldrich.com for staining cells with TMS-008. The following information does appear on the emdmillipore.com website:
Product is presented as a 40mM solution at pH 4.1 +/- 0.1.As the molecular weight is 342.26, the percentage of Alizarin Red should be 1.4%. Most of the common histology textbooks generally recommend using a 2% concentration of Alizarin Red. But as in many histology procedures, the exact percentage is generally not critical for acceptable staining. It would appear the developers of the solution thought they preferred a concentration of 1.4%. This is typical of many histological staining procedures. Methods can and do vary from source to source.
As to a protocol, protocols for most histology textbooks should be acceptable for use. In general, tissue should not be fixed in fixative that contain calcium. Fixatives commonly used as 10% Neutral Buffered Formalin or alcoholic formalin. Once the slides have been prepared, hydrate sections in 50% alcohol. Then rinse briefly with distilled water. Incubate the slides in the Alizarin Red solution anywhere from 30 second to 5 minutes or more. Check for stain development every 30 seconds under a microscope until a red lake forms. The lake should not be too diffuse and on the heavy side. Then remove the solution by shaking off the excess and blot dry. Flood slides with acetone for about 20 seconds. Use a 50:50 mixture of acetone and xylene to differentiate. Clear in xylene. If possible, mount with a synthetic mounting media.
The solution does not have great stability. The website further states: Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
This product is included in some of our kits, such as ECM815 or ECM810. Here are the user guides for the kit ECM815 and ECM810, which contains information about the Alizarin red solution protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/190/283/ecm810man.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/119/ecm815.pdfYou can also find application information for Alizarin Red at the following links:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/stem-cell-culture/mesenchymal-stem-cell-culture-protocolsHelpful?
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