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Merck
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SCM075

Sigma-Aldrich

ROCK 抑制剂（Y-27632）

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

别名:

Y-27632

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32160801
NACRES:
NA.75

品質等級

100

形狀

liquid

分子量

320.26 g/mol

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

相关类别

一般說明

ROCK抑制剂（Y-27632）是包括p160Rock（Ki=140nM）和Rock-II在内的Rho相关蛋白激酶（ROCK）的一种选择性ATP竞争性抑制剂。还可作为肌球蛋白磷酸化和平滑肌收缩的激动剂诱导的Ca2+敏化的一种有效抑制剂。用10uM Y-27632处理一小时后可阻断已发生解离的培养的人类胚胎干细胞（hESC）的凋亡，从而在不影响多能性的情况下提高hESC的存活率和克隆效率1。近期有报告表明Y-27632和Accutase（货号SCR005）所组成的消化酶混合配方，能够通过使用常规的慢速冷冻和快速解冻方法提高单个hESC的解冻后存活率

儲存和穩定性

在重悬前：维持在-20ºC避光保存2年。在重悬后：在-20ºC避光保存1年

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Malgorzata Bienkowska-Haba et al.
Journal of virology, 94(3) (2019-11-22)
It is established that the host cell transcriptomes of natural lesions, organotypic rafts, and human papillomavirus (HPV)-immortalized keratinocytes are altered in the presence of HPV genomes. However, the establishment of HPV-harboring cell lines requires selection and immortalization, which makes it
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Ambra Gianotti et al.
Frontiers in pharmacology, 9, 1176-1176 (2018-11-13)
Cystic fibrosis (CF) is a chronic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, which encodes for a channel expressed at the apical surface of epithelial tissues. Defective chloride and bicarbonate secretion, arising from CFTR mutations
Marta Słoniecka et al.
Journal of molecular medicine (Berlin, Germany), 97(10), 1477-1489 (2019-08-11)
Fibrosis is characterized by hardening, overgrowth, and development of scars in various tissues as a result of faulty reparative processes, diseases, or chronic inflammation. During the fibrotic process in the corneal stroma of the eye, the resident cells called keratocytes
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
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