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product name
HL-1 心肌细胞系, HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.
生物源
mouse
品質等級
技術
cell based assay: suitable
cell culture | mammalian: suitable
一般說明
HL-1心肌细胞系是永生化的小鼠心肌细胞系,能够连续分裂并自发收缩,同时保持分化的心脏表型。 HL-1可以连续传代,而不会失去其分化的心肌细胞表型,包括形态、生化和电生理特性。HL-1细胞已用于研究正常心肌细胞在信号传导、电、代谢和转录调控方面的功能,以及解决诸如缺氧、高血糖-高胰岛素血症、细胞凋亡和缺血再灌注等病理状况。
HL-1系源自从成年雌性C57BL/6J小鼠切除的AT-1皮下肿瘤。亲本AT-1系最初源自在转基因小鼠中生长的心房肿瘤,其中SV40大T抗原的表达通过心房利钠因子(ANF)启动子靶向心房心肌细胞。
HL-1系源自从成年雌性C57BL/6J小鼠切除的AT-1皮下肿瘤。亲本AT-1系最初源自在转基因小鼠中生长的心房肿瘤,其中SV40大T抗原的表达通过心房利钠因子(ANF)启动子靶向心房心肌细胞。
細胞系描述
心肌细胞
應用
根据产品文件中详述的“学术使用协议”条款,本产品预期仅用于销售和销售给学术机构,以供内部学术研究使用。有关任何其他用途的信息,请联系licensing@emdmillipore.com。
研究类别
心脏
心血管疾病
毒性
心脏
心血管疾病
毒性
品質
• 每瓶含有≥ 1X106 个活细胞。
•Charles River动物诊断服务通过小鼠必要透明面板PCR面板对细胞进行传染病检测,结果为阴性。
•细胞对支原体污染呈阴性。
•功能测试:基于Fluo-8钙染料的荧光检测细胞内钙的变化
•Charles River动物诊断服务通过小鼠必要透明面板PCR面板对细胞进行传染病检测,结果为阴性。
•细胞对支原体污染呈阴性。
•功能测试:基于Fluo-8钙染料的荧光检测细胞内钙的变化
儲存和穩定性
HL-1心肌细胞系应保存在液氮中。在初始解冻后,细胞可以培养至少10代,而不会显著影响细胞标志物的表达和功能。
免責聲明
除非在产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得对人或动物用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用。
本产品含有转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。这项法律规定{HCompany}有义务要求您提供某些信息,以及有关处理 GMO 的机构的信息。请点击这里,获得最终用户声明(EUD)表格。
本产品含有转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。这项法律规定{HCompany}有义务要求您提供某些信息,以及有关处理 GMO 的机构的信息。请点击这里,获得最终用户声明(EUD)表格。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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