The Rat Stress Hormone panel was tested with 8 mouse samples (2 each of OB/OB, DB/DB, Swiss Webster, and CD-1), and the analytes in the samples tested were successfully detected. However, it's important to note that the assay conditions were not fully validated or optimized for mice. Therefore, the assay need to be validated specifically for mouse samples.
RSHMAG-69K
MILLIPLEX® 大鼠应激激素磁珠面板-内分泌多重检测
This MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel is an Endocrine multiplex assay. The analytes available for this multiplex kit are: ACTH, Corticosterone, Melatonin.
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About This Item
UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
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质量水平
种属反应性
rat
制造商/商品名称
Milliplex®
assay range
accuracy: 61%
(Corticosterone)
accuracy: 83%
(ACTH)
sensitivity: 0.9 pg/mL
(ACTH)
sensitivity: 1.52 pg/mL
(Melatonin)
sensitivity: 2914 pg/mL
(Corticosterone)
standard curve range: 0.55-400 pg/mL
(Melatonin)
standard curve range: 1.4-1,000 pg/mL
(ACTH)
standard curve range: 686-500,000 pg/mL
(Corticosterone)
inter-assay cv: <15%
intra-assay cv: <10%
技术
multiplexing: suitable
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
应激被定义为通过生理和/或心理刺激或应激源破坏体内稳态。在生理上，包括下丘脑、垂体和肾上腺在内的神经内分泌系统在应激反应中起关键作用。在促肾上腺皮质激素释放激素（CRH）和精氨酸加压素（AV）的激活下，垂体释放ACTH，导致肾上腺分泌皮质醇/皮质酮和其他糖皮质激素。这些皮质激素在应激反应中涉及全身，并通过对下丘脑的负反馈回路参与应激反应的终止。皮质酮和褪黑激素在调节睡眠-觉醒周期中很重要。
MILLIPLEX® 大鼠应激激素面板是一种3重试剂盒，用于同时定量血清、血浆、组织/细胞提取物或培养基样品中的以下任何或所有分析物：ACTH，皮质酮和/或褪黑激素。该试剂盒采用96孔板，包含冻干标准混合物、两个内部检测质控品，最多可测量38份样本，一式两份。
Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省有价值的样品量。
面板类型：内分泌
MILLIPLEX® 大鼠应激激素面板是一种3重试剂盒，用于同时定量血清、血浆、组织/细胞提取物或培养基样品中的以下任何或所有分析物：ACTH，皮质酮和/或褪黑激素。该试剂盒采用96孔板，包含冻干标准混合物、两个内部检测质控品，最多可测量38份样本，一式两份。
Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省有价值的样品量。
面板类型：内分泌
特异性
交叉反应性
此检测试剂在检测板内显示不显著的交叉反应性
此检测试剂在检测板内显示不显著的交叉反应性
应用
- 分析物：ACTH、皮质酮、褪黑激素
- 推荐样品类型：大鼠血清、血浆、细胞/组织上清液和裂解物
- 注：为了获得浑浊的血清和血浆样品的最佳分析性能，提供了提取方案。
- 建议的样品稀释度：每孔25 μL用于以下方面：未稀释的提取血清或血浆；1:4未提取的血清或血浆；细胞/组织培养样品可能需要在适当的对照培养基中稀释。
- 分析运行时间：在2-8℃下过夜（16-18小时）
- 研究类别：内分泌
- 研究子类别：代谢
特点和优势
通过在此面板中选择可用的分析物来设计多重试剂盒。
其他说明
请联系技术服务部了解稀释线性。
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Is it possible to use the Milliplex Rat Stress Hormone Panel (RSHMAG-69K) to detect hormones in mouse samples?
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