MABS829

Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2

clone 3E7.2, from mouse

• 别名: Epidermal growth factor receptor, EC: 2.7.10.1, Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: 3E7.2, monoclonal
• 种属反应性: human
• 技术: immunocytochemistry: suitable; western blot: suitable
• 同位素/亚型: IgG2bκ
• NCBI登记号: NP_005219
• UniProt登记号: P00533
• 运输: ambient
• 靶向翻译后修饰: phosphorylation (pTyr1173)
• 基因信息: human ... EGFR(1956)

说明

一般描述

Epidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1; Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is activated by binding of its specific ligands and transforms from a monomeric to active homodimeric form. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine residues (Y992, Y1045, Y1068, Y1148 and Y1173) occurs in the C-terminal domain. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding domains. EGFR activation results in at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. These downstream events modulate several cell functions, including migration, adhesion, and proliferation. Endocytosis and inhibition of the activated EGFR by phosphatases constitute key regulatory mechanisms.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids surrrounding Tyrosine 1173 of human epidermal growth factor receptor.

应用

Detect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected phospho-EGFR (Tyr1173) in A431 cells treated with EGF.

质量

Evaluated by Western Blotting in A431 cells treated with EGF.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 µg lysate from A431 cells treated with EGF.

目标描述

~200 kDa observed; 134.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 05-483

外形

Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1