MABS1145

Anti-3-PGDH Antibody, clone TK-08 13A8

clone TK-08 13A8, from mouse

• 别名: D-3-phosphoglycerate dehydrogenase, 3-PGDH
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: TK-08 13A8, monoclonal
• 种属反应性: human
• 技术: immunohistochemistry: suitable; western blot: suitable
• 同位素/亚型: IgG1κ
• NCBI登记号: NP_006614
• UniProt登记号: O43175
• 运输: dry ice
• 靶向翻译后修饰: unmodified
• 基因信息: human ... PHGDH(26227)

说明

一般描述

D-3-phosphoglycerate dehydrogenase (EC1.1.1.95; UniProt O43175; also known as 3-PGDH, Epididymis secretory protein Li 113) is encoded by the PHGDH (also known as 3PGDH, HEL-S-113, NLS, NLS1, PGAD, PGDH, PDG, PGD, PGDH3, PHGDHD, SERA) gene (Gene ID 26227) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. L-serine is an important precursor for macromolecule biosynthesis. 3-PGDH catalyzes the first and rate-limiting step of the serine synthesis pathway from 3-phosphoglycerate to phospho-hydroxypyruvate and is important during tumor development. The PHGDH gene is frequently amplified and the enzyme is overexpressed in 70% of the estrogen receptor-negative breast cancers, 39% of melanoma, and cervical cancers.

免疫原

Recombinant protein corresponding to human 3-PGDH.

应用

Detect PHGDH using this mouse Monoclonal Anti-3-PGDH Antibody, clone TK-08 13A8, Cat. No. MABS1145, validated for use in Western Blotting.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected 3-PGDH in 10 µg HEK293 and Raji cell lysates.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected 3-PGDH in human salivary gland tissue sections.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 3-PGDH in 10 µg of HeLa cell lysate.

目标描述

~57 kDa observed

外形

Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable