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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
PS 45, monoclonal
种属反应性
mouse, human, bovine, frog, rat
技术
ELISA: suitable
electron microscopy: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
同位素/亚型
IgG2bκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
rat ... Avp(24221)
一般描述
The neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormons oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormon peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Posttranslational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an antidiuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
特异性
Clone PS 45 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1.
免疫原
Epitope: Near Neurophysin 2 C-terminus
应用
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat brain and rat pituitary tissue.
Radioimmunoassay (RIA): Target specificity of a representative lot was confirmed by RIA. Only rat neurophysin 2, but not neurophysin 1, oxytocin, or angiotensin II, can compete against 125I-neurophysin 2 for binding mAb PS 45 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy (EM): A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
3. References/ Please add reference:
Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97
Radioimmunoassay (RIA): Target specificity of a representative lot was confirmed by RIA. Only rat neurophysin 2, but not neurophysin 1, oxytocin, or angiotensin II, can compete against 125I-neurophysin 2 for binding mAb PS 45 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy (EM): A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
3. References/ Please add reference:
Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97
This Anti-Neurophysin 2/NP-AVP Antibody, clone PS 45 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Radioimmunoassay, Electron Microscopy, Immunoprecipitation, ELISA for the detection of Neurophysin 2/NP-AVP.
质量
Evaluated by Western Blotting in rat pituitary tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
目标描述
~15 kDa observed
外形
Format: Purified
其他说明
Concentration: Please refer to lot specific datasheet.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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