MABN2621

Anti-UNC119 homolog A/mRG4 Antibody, clone A2

• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: mouse
• 质量水平: 200
• 偶联物: unconjugated
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: A2, monoclonal
• 分子量: calculated mol wt 27.01 kDa; observed mol wt ~28 kDa
• 纯化方式: using protein G
• 种属反应性: mouse
• 包装: antibody small pack of 100 μg
• 技术: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同位素/亚型: IgG1κ
• 表位序列: Unknown
• Protein ID登记号: NP_035806
• UniProt登记号: Q9Z2R6
• 运输: 2-8°C
• 靶向翻译后修饰: unmodified
• 基因信息: mouse ... Unc119(22248)

说明

一般描述

Protein unc-119 homolog A (UniProt: Q9Z2R6; also known as Retinal protein 4, mRG4) is encoded by the Unc119 (also known as Unc119h) gene (Gene ID: 22248) in murine species. UNC119 is an adaptor protein that is involved in the development of the vertebrate nervous system. It is widely expressed among vertebrates and invertebrates and is predominantly localized in photoreceptor synapses in the outer plexiform layer of the retina. It recognizes the acylated N-terminus of the rod photoreceptor transducin -subunit (T ). Apart from its presence in the mammalian retina, it is also detected in leukocytes, T-cells, lung fibroblasts, the adrenal glands, cerebellum, and kidney. UC119 is reported to activate Src-type tyrosine kinases associated with the interleukin-5 receptor and the T-cell receptor by interacting with Src homology domains localized in its N-terminal half. It is also reported to stimulate the induction of -smooth muscle actin and myofibroblast differentiation by TGF-β in human lung fibroblasts. UNC119 can undergo phosphorylation at serine 37, 39, and 41 by casein kinase 2 and these phosphorylations suppresses its interaction with KLHL18 (Kelch Like Family Member 18) and downregulates its KLHL18-mediated degradation. (Ref.: Chaya, T., et al. (2019). EMBO J. 38(23); e101409; Zhang, H., et al. (2011). Nat. Neurosci. 14(7); 874-880; Vepachedu, R., et al. (2007). J. Immunol. 179(1); 682-690).

特异性

Clone A2 is a mouse monoclonal antibody that detects murine UNC119 Homolog A protein (mRG4).

免疫原

His-tagged full-length recombinant mouse UNC119 homolog A (mRRG4).

应用

Quality Control Testing

Evaluated by Western Blotting in Mouse retina tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UNC119 homolog A (mRG4) in Mouse retina tissue lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected UNC119 homolog A/mRG4 in Immunofluorescence applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci. 49(6):2366-75).

Western Blotting Analysis: A representative lot detected UNC119 homolog A/mRG4 in Western Blotting applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci. 49(6):2366-75).

Immunohistochemistry Applications: A representative lot detected UNC119 homolog A/mRG4 in Immunohistochemistry applications (Haeseleer, F., et al. (2008). Invest Ophthalmol Vis Sci.;49(6):2366-75).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-UNC119 homolog A/mRG4, clone A2, Cat. No. MABN2621, is a mouse monoclonal antibody that detects UNC119 homolog A (mRG4) and is tested for use in Immunohistochemistry, Immunofluorescence, and Western Blotting.

外形

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1