MABD151

Anti-podocalyxin associated keratan sulfate, clone R-10G| MABD151 Antibody

clone R-10G, from mouse

• 别名: Podocalyxin, PC, PCLP-1
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: R-10G, monoclonal
• 种属反应性: human
• 请勿与下列物质发生反应: mouse
• 技术: immunocytochemistry: suitable; western blot: suitable
• 同位素/亚型: IgG1, kappa
• NCBI登记号: NP_001018121
• UniProt登记号: O00592
• 运输: wet ice
• 靶向翻译后修饰: unmodified
• 基因信息: human ... PODXL(5420)

说明

一般描述

Podocalyxin-like protein 1 is a sialomucin that is involved in the regulation of both cell adhesion and cell morphology through its interactions with several intracellular and at least one extracellular ligand. It is known to play an essential role in the formation and maintenance of podocyte foot processes, in cancer development and aggressiveness and as a marker of undifferentiatted embryonic stem cells. MABD151, a monoclonal antibody generated against human iPS cells, is a novel marker antibody recognizing a type of keratan sulfate lacking oversulfated structure on hiPS cells. MABD151 is a useful molecular probe for disclosing the roles of glycans on the surface of hiPS cells in the maintenance of self-renewal and pluripotency and also as a potent tool for the evaluation and standardization of hiPS cells with different tissue origins and different histories in regenerative medicine (Kawabe K et.al, (2013) Glycobiology. v23, 322–336.

免疫原

Whole Human iPS cells (Tic cell line).

应用

Evaluated by Western Blot in human brain lysate.
Western Blot Analysis: 1µg/mL of this antibody detected podocalyxin associated keratin sulphate in 10 µg of human brain lysate.

Evaluated by Immunocytochemistry in human ES cells.
Immunocytochemical Analysis: A 1:250 dilution of this antibody detected odocalyxin associated keratin sulphate in human ES cells.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-podocalyxin associated keratan sulfate antibody, clone R-10G is validated for use in ICC & western blotting for the detection of podocalyxin associated keratan sulfate.

质量

Evaluated by Western Blot in human ES cell lysate.

Western Blot Analysis: 1µg/mL of this antibody detected podocalyxin associated keratin sulphate in 10 µg of human ES cell lysate.

目标描述

above 250kDa

外形

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable